Rescue of Low-Yield DNA Samples for Next-Generation Sequencing Using Vacuum Centrifugal Concentration in a Clinical Workflow

The implementation of next-generation sequencing (NGS) in clinical oncology has enabled the analysis multiple cancer-associated genes for diagnostics and treatment purposes. detection pathogenic likely mutations is crucial to manage disease. Obtaining mutational profile may be challenging samples with low yields DNA—reflected by type biological material, such as formalin-fixed paraffin-embedded tissue (FFPE), needle biopsies, circulating free/tumor DNA, well a sparse tumor content. Moreover, standardized strict procedures extraction DNA setting might contribute lower amounts per µL. variants low-yield remains challenge diagnostics, where molecular analyses NGS are needed. Here, we performed vacuum centrifugation on extracted from five FFPE blocks, concentrations below 0.2 ng/µL. Through analysis, found that could concentrated sufficient levels, without compromising profile.